TESA & Micro-TESE: Advanced Sperm Retrieval for Male Infertility
Advanced Solutions for Azoospermia. Expert Male Fertility Microsurgery at 6venus Fertility & Urology, Pune.
What is TESA and Micro-TESE?
TESA (Testicular Sperm Aspiration): This is a simple, needle-based procedure typically used for Obstructive Azoospermia. If sperm is being produced normally but is blocked (due to previous surgeries, infections, or congenital issues), Dr. Bendale uses a fine needle to gently aspirate sperm directly from the testicular tissue. It is quick, minimally invasive, and requires no incisions.
Micro-TESE (Microsurgical Testicular Sperm Extraction): This is the “Gold Standard” for Non-Obstructive Azoospermia, where sperm production is severely low. Using a high-powered operating microscope, Dr. Bendale meticulously examines the seminiferous tubules. He identifies the “fuller,” healthier-looking tubules that are most likely to contain sperm. This microscopic precision allows us to find sperm even where a standard biopsy might fail.
TESA vs. Micro-TESE: Which one do you need?
Success depends on choosing the right procedure for your specific condition.
TESA (Testicular Sperm Aspiration): A simple, needle-based procedure used when sperm is being produced but is blocked from exiting (Obstructive Azoospermia).
Micro-TESE (Microsurgical Testicular Sperm Extraction): The “Gold Standard” for Non-Obstructive Azoospermia. Using a high-powered operating microscope, Dr. Bendale identifies the most promising “full” tubules to find sperm where others cannot.
Precision is our priority. While TESA is a quick, 10-minute solution for blockages, Micro-TESE is a meticulous “search and rescue” mission that increases the chances of finding sperm by up to 50% in difficult cases. By utilizing high-magnification optics at our Wakad center, we ensure that only the most viable tissue is sampled, preserving your hormonal health. At 6venus, our goal is to achieve the highest retrieval rate with the least possible impact on your body, staying true to our “1% natural” recovery philosophy.
Restoring Hope for Fatherhood
When a semen analysis shows zero sperm count (Azoospermia), it doesn’t mean biological fatherhood is impossible. At 6venus, we specialize in advanced sperm retrieval techniques that bypass obstructions or production issues directly at the source.
Led by Dr. Pavan Bendale (M.B.B.S., DGO, DNB), our urology wing uses high-precision surgical methods to find and retrieve sperm, even in the most challenging cases. We bridge the gap between “no sperm” and a successful pregnancy.
Why Choose us for TESA & Micro-TESE?
Success in cryopreservation depends on the Vitrification technology and the safety of the storage facility.
Dual Expertise
Led by Dr. Pavan Bendale (M.B.B.S., DGO, DNB), our team combines 10+ years of urological surgery with advanced fertility science.
High-Powered Microscopy
We use advanced surgical microscopes to increase retrieval rates while minimizing tissue damage.
On-Site Embryology
The moment tissue is retrieved, it is handed to our lab to confirm sperm presence, reducing the time you are under anesthesia.
The TESA/Micro-TESE Path
Advanced surgical precision by Dr. Pavan Bendale
Diagnostic Screening
A detailed hormonal profile and Scrotal Doppler are performed to determine if the cause is Obstructive or Non-Obstructive.
Pre-Op Planning
Dr. Bendale discusses the procedure type (TESA or Micro-TESE) based on your unique fertility audit and ultrasound findings.
The Procedure
Performed under local or general anesthesia. Using micro-instruments, sperm is extracted with minimal tissue impact.
On-Site Lab Check
The retrieved tissue is immediately checked by our embryologists to confirm the presence of healthy, motile sperm.
Sperm Cryo-Storage
Once retrieved, the sperm is frozen (vitrified) so it can be used for future IVF/ICSI cycles without needing another surgery.
Post-Op Recovery
A quick recovery time. Most patients return to light activities in 48 hours with Dr. Bendale's personalized care plan.
Frequently Asked Questions
TESA/Micro TESE
Is the procedure painful, and what kind of anesthesia is used?
At 6venus, your comfort is our priority. TESA is usually performed under local anesthesia or mild sedation, and most men describe it as a minor “pinch” or pressure. Micro-TESE is a more detailed microsurgery, so it is performed under general anesthesia or deep sedation. This ensures you are completely asleep and feel no pain during the 1–2 hour procedure. Post-surgery, you may feel some dull aching or soreness, which is easily managed with the personalized pain-relief plan provided by Dr. Pavan Bendele.
If sperm is found during TESA/Micro-TESE, can we use it for IUI?
This is a common question. Unfortunately, surgically retrieved sperm are usually fewer in number and do not have the same “swimming ability” (motility) as naturally ejaculated sperm. Because of this, they cannot travel through the cervix and uterus effectively on their own. Therefore, these sperm must be used with ICSI (Intracytoplasmic Sperm Injection), where a single healthy sperm is injected directly into the egg in our lab to ensure the highest chance of fertilization.
What happens if no sperm is found during the surgery?
We believe in complete transparency. While Micro-TESE has the highest success rate for finding sperm (up to 60% in many cases), there is a chance that no viable sperm are found. Before the surgery, Dr. Pavan Bendele will discuss “Plan B” with you. This might include using donor sperm (if previously agreed upon) or discussing further medical therapies to improve production before a future attempt. Having an on-site lab allows us to give you a definitive answer while you are still in the facility.
Will this surgery affect my testosterone levels or sexual function?
This is a major concern for many men. Micro-TESE is specifically designed to be “tissue-sparing.” Because Dr. Bendele uses a high-powered microscope, he only removes the tiny tubules that appear healthy, rather than taking large chunks of tissue. This precision protects the Leydig cells (which produce testosterone). Most studies show that testosterone levels remain stable or return to normal shortly after the procedure, and there is no long-term impact on sexual function or libido.
How should I prepare my body to increase the chances of finding sperm?
Success starts long before the surgery. Dr. Bendele often recommends a 3-month “1% Natural” preparation phase. Since it takes about 72–90 days for new sperm to mature, we suggest:
Stopping smoking and limiting alcohol.
Avoiding “heat” (no saunas, hot tubs, or tight underwear).
Taking specific antioxidant supplements prescribed at our Pune clinic to reduce DNA fragmentation.
Managing stress and improving sleep, which directly impacts the hormones that trigger sperm production.
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